FEBS Letters | |
Cloning and expression of a cDNA for the rat k‐opioid receptor | |
Satoh, Masamichi1  Maekawa, Keiko2  Kaneko, Shuji2  Minami, Masabumi2  Toya, Takashi2  Katao, Yoshikazu1  Nakamura, Shunya2  Onogi, Tatsuhiro2  | |
[1] Molecular Pharmacology, Faculty of Pharmaceutical Sciences, Kyoto University, Kyoto 606-01, Japan;Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kyoto University, Kyoto 606-01, Japan | |
关键词: Opioid receptor; k-Type; Dynorphin A; U50488H; PCR; polymerase chain reaction; RT; reverse transcription; DPDPE; [d-Pen2 d-Pen5enkephalin; DAMGO; [d-Ala; MePhe4; Gly(ol)5]enkephalin; G protein; GTP binding protein; | |
DOI : 10.1016/0014-5793(93)80240-U | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
We cloned a cDNA for the rat K-opioid receptor from a rat thalamus cDNA library. The deduced amino acid sequence consists of 380 residues with features shared by members of the G protein-coupled receptor family. The specific binding of [3H]bremazocine to the membrane of COS-7 cells transfected with the cDNA was displaced by k-specific opioid ligands, but not by μ- and δ-specific ligands. Xenopus oocytes injected with the in vitro transcribed mRNA responded to opioid ligands with the same subtype specificity. Northern blot analysis demonstrated that k-opioid receptor mRNA is expressed in a regionally specific manner in rat brain.
【 授权许可】
Unknown
【 预 览 】
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RO201912020298358ZK.pdf | 504KB | download |