Cloning and expression of a cDNA for the rat k‐opioid receptor

FEBS Letters

Satoh, Masamichi¹  Maekawa, Keiko²  Kaneko, Shuji²  Minami, Masabumi²  Toya, Takashi²  Katao, Yoshikazu¹  Nakamura, Shunya²  Onogi, Tatsuhiro² 

[1] Molecular Pharmacology, Faculty of Pharmaceutical Sciences, Kyoto University, Kyoto 606-01, Japan;Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kyoto University, Kyoto 606-01, Japan

关键词: Opioid receptor;    k-Type;    Dynorphin A;    U50488H;    PCR;    polymerase chain reaction;    RT;    reverse transcription;    DPDPE;    [d-Pen2 d-Pen5enkephalin;    DAMGO;    [d-Ala;    MePhe4;    Gly(ol)5]enkephalin;    G protein;    GTP binding protein;   

DOI  :  10.1016/0014-5793(93)80240-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We cloned a cDNA for the rat K-opioid receptor from a rat thalamus cDNA library. The deduced amino acid sequence consists of 380 residues with features shared by members of the G protein-coupled receptor family. The specific binding of [³H]bremazocine to the membrane of COS-7 cells transfected with the cDNA was displaced by k-specific opioid ligands, but not by μ- and δ-specific ligands. Xenopus oocytes injected with the in vitro transcribed mRNA responded to opioid ligands with the same subtype specificity. Northern blot analysis demonstrated that k-opioid receptor mRNA is expressed in a regionally specific manner in rat brain.

【 授权许可】

Unknown