Cloning of a human liver UDP‐glucose pyrophosphorylase cDNA by complementation of the bacterial galU mutation

FEBS Letters

Chang, Hwan-You²  Peng, Hwei-Ling¹ 

[1] Department of Microbiology and Immunology, Chang-Gung Medical College, Kwei-San, Taiwan, ROC;Department of Molecular and Cellular Biology, Chang-Gung Medical College, Kwei-San, Taiwan, ROC

关键词: E. coli galU;    Glycogen;    UDP-glucose pyrophosphorylase cDNA;   

DOI  :  10.1016/0014-5793(93)80213-E

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A human liver cDNA clone which encodes the UDP-glucose pyrophosphorylase was isolated by complementation of a bacterial galU mutant. The deduced amino acid sequence of the human enzyme comprised 508 amino acids with a calculated molecular mass of 56,950. The human enzyme significantly resembles those of potato tuber and slime mold with a homology of 46.6% and 43.2%, respectively, in amino acid sequence. No homology was found between the eukaryotic and the prokaryotic enzymes. Northern blotting analysis revealed that the gene was expressed at the highest level in skeletal muscle, followed by liver, heart and kidney.

【 授权许可】

Unknown