Chelatable iron and copper can be released from extracorporeally circulated blood during cardiopulmonary bypass

FEBS Letters

Quinlan, Gregory J.¹  Evans, Timothy E.¹  Moat, Neil E.¹  Gutteridge, John M.C.¹ 

[1] Oxygen Chemistry Laboratory, Unit of Critical Care, Royal Brompton Hospital and Natlonal Heart & Lung Institute, Sydney Street, London, SW36NP, UK

关键词: Bypass surgery;    Oxygen radical;    Bleomycin-detectable iron;    Phenanthroline-detectable copper;    Reoxygenation injury;    Antioxidants;    Elastase;    Caeruloplasmin;    Haemoglobin;   

DOI  :  10.1016/0014-5793(93)80974-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

During cardiopulmonary bypass surgery blood is extracorporeally oxygenated and circulated before returning to the systemic arterial circulation. Blood undergoing extracorporeal dilution and circulation is exposed to non-physiological surfaces, which cause the activation of several regulatory cascades. Cells are also subjected to damaging shear stresses. Under such conditions neutrophils can be ‘activated’ to release reactive oxygen intermediates such as O₂ ⁻ and H₂O₂, and other cells can release proteolytic enzymes and metalloproteins. Collectively, these events can result in the release of micromolar quantities of redox active iron and copper. Bleomycin-detectable iron and phenanthroline-detectable copper were found in two out of four mock bypass experiments. However, there was no correlation between the presence of chelatable iron and copper and the activation of neutrophils measured as elastase.

【 授权许可】

Unknown