Monensin and forskolin inhibit the transcription rate of sucrase‐isomaltase but not the stability of its mRNA in Caco‐2 cells

FEBS Letters

Swallow, D.²  Lacasa, M.¹  Rousset, M.³  Chantret, I.³  Chevalier, G.³ 

[1] IRSC Laboratoire Virus et Différenciation, 7 rue Guy Moquet, 94800 Villejuif, France and Université Pierre et Marie Curie, 4 Place Jussieu, 75005 Paris, France;MRC Human Biochemical Genetics Unit, The Galton Laboratory, University College London, 4 Stephenson Way, London, NWl 2HE, UK;Unité de Recherches sur la Différenciation Cellulaire Intestinale, INSERM U178, 16 avenue Paul-Vaillant Couturier, 94807 Villejuif Cedex, France

关键词: Sucrase-isomaltase;    Caco-2 cell;    Forskolin;    Monensin;    Transcription;    Glucose repression;   

DOI  :  10.1016/0014-5793(93)80964-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Treatment of Caco-2 cells with forskolin (25 μM) or monensin (1 μM) has previously been shown to cause a marked decrease in the level of sucrase-isomaltase (SI) mRNA, without any effect on the expression of dipeptidylpeptidase IV (DPP-IV). In the present work, we report that there is no significant difference in the stability of SI mRNA between control and treated cells. On the other hand, we demonstrate a decrease in the transcription rate of SI mRNA which is sufficient to account for the decrease in the steady-state level of SI mRNA both in forskolin- and monensin-treated Caco-2 cells.

【 授权许可】

Unknown