Spleen‐derived growth factor, SDGF‐3, is identified as keratinocyte growth factor (KGF)

FEBS Letters

Osada, Hiroyuki¹  Suzuki, Tohru⁶  Aaronson, Stuart A.⁴  Itoh, Takehiro²  Mitsui, Youji²  Koga, Nobumitsu⁵  Rubin, Jeffrey S.⁴  Suzuki, Masashi²  Saito, Takao³ 

[1] Antibiotics Laboratory, The Institute of Physical and Chemical Research (RIKEN), Wako, Saitama 305-01, Japan;Cell Biology Laboratory, National Institute of Bioscience and Human-Technology, Higashi 1-1 Tsukuba Science City, Ibaraki 305, Japan;Material Chemistry Division, Government Industrial Research Institute, Nagoya, Hirate 1, kita, Nagoya 462, Japan;Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, MD 20892, USA;Advanced Technology Laboratory, Mayekawa MFG. Co., Ltd., Moriya-machi, Ibaraki 302-01, Japan;Department of Biotechnology, Faculty of Agriculture, Gifu University, Yanago, Gifu 501-11, Japan

关键词: SDGF-3;    Keratinocyte growth factor;    Bovine spleen;    Primary cultured hepatocyte;    Heparin binding;    KGF;    keratinocyte growth factor (also designated FGF-7);    SDGF-3;    spleen derived growth factor-3;    EGF;    epidermal growth factor;    TGF-a;    transforming growth factor-a;    FGF-1;    fibroblast growth factor-1 (acidic FGF);    FGF-2;    fibroblast growth factor-2(basic FGF);    HGF/SF;    hepatocyte growth factor/scatter factor;    CHAPS;    3-[3-cholamidopropyl)dimethylammonio]1-propanesulfonate;    SDS-PAGE;    sodium dodecyl sulfate-polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(93)80956-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A heparin-binding mitogen rat for rat hepatocytes was partially purified from bovine spleen by a combination of heparin-affinity, cation-exchange and gel-filtration chromatography. Besides stimulating rat hepatocytes, this factor, which was designated spleen-derived growth factor-3 (SDGF-3), exhibited mitogenic activity for mouse epidermal keratinocytes but not mouse fibroblasts. Its apparent epithelial specificity and heparin-binding properties corresponded to those of keratinocyte growth factor (KGF). These findings, together with the fact that the mitogenic activity of SDGF-3 was abolished by a neutralizing monoclonal antibody specific for KGF, identify this bovine spleen-derived hepatocyte mitogen as KGF.

【 授权许可】

Unknown