FEBS Letters | |
Spleen‐derived growth factor, SDGF‐3, is identified as keratinocyte growth factor (KGF) | |
Osada, Hiroyuki1  Suzuki, Tohru6  Aaronson, Stuart A.4  Itoh, Takehiro2  Mitsui, Youji2  Koga, Nobumitsu5  Rubin, Jeffrey S.4  Suzuki, Masashi2  Saito, Takao3  | |
[1] Antibiotics Laboratory, The Institute of Physical and Chemical Research (RIKEN), Wako, Saitama 305-01, Japan;Cell Biology Laboratory, National Institute of Bioscience and Human-Technology, Higashi 1-1 Tsukuba Science City, Ibaraki 305, Japan;Material Chemistry Division, Government Industrial Research Institute, Nagoya, Hirate 1, kita, Nagoya 462, Japan;Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, MD 20892, USA;Advanced Technology Laboratory, Mayekawa MFG. Co., Ltd., Moriya-machi, Ibaraki 302-01, Japan;Department of Biotechnology, Faculty of Agriculture, Gifu University, Yanago, Gifu 501-11, Japan | |
关键词: SDGF-3; Keratinocyte growth factor; Bovine spleen; Primary cultured hepatocyte; Heparin binding; KGF; keratinocyte growth factor (also designated FGF-7); SDGF-3; spleen derived growth factor-3; EGF; epidermal growth factor; TGF-a; transforming growth factor-a; FGF-1; fibroblast growth factor-1 (acidic FGF); FGF-2; fibroblast growth factor-2(basic FGF); HGF/SF; hepatocyte growth factor/scatter factor; CHAPS; 3-[3-cholamidopropyl)dimethylammonio]1-propanesulfonate; SDS-PAGE; sodium dodecyl sulfate-polyacrylamide gel electrophoresis; | |
DOI : 10.1016/0014-5793(93)80956-U | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
A heparin-binding mitogen rat for rat hepatocytes was partially purified from bovine spleen by a combination of heparin-affinity, cation-exchange and gel-filtration chromatography. Besides stimulating rat hepatocytes, this factor, which was designated spleen-derived growth factor-3 (SDGF-3), exhibited mitogenic activity for mouse epidermal keratinocytes but not mouse fibroblasts. Its apparent epithelial specificity and heparin-binding properties corresponded to those of keratinocyte growth factor (KGF). These findings, together with the fact that the mitogenic activity of SDGF-3 was abolished by a neutralizing monoclonal antibody specific for KGF, identify this bovine spleen-derived hepatocyte mitogen as KGF.
【 授权许可】
Unknown
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