Glutathione, but not glutamine, is detected in 13C‐NMR spectra of perchloric acid extracts from C6 glioma cells

FEBS Letters

Portais, Jean-Charles¹  Canioni, Paul¹  Merle, Michel¹  Martin, Magali¹ 

[1] Département de Résonance Magnétique, Institut de Biochimie Cellulaire du CNRS et Université de Bordeaux II, 1, rue C. Saint-Saëns, 33077 Bordeaux-Cedex, France

关键词: C6 cell;    Glutathione;    Glutamine;    13C-NMR spectroscopy;    DMEM;    Dulbecco's modified Eagle's medium;    GSH;    glutathione;    PBS;    phosphate-buffered saline;    GFAP;    glial fibrillary acidic protein;   

DOI  :  10.1016/0014-5793(93)81009-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Glutamine, which is expected to be produced by C6 glioma cells, is not detected in both amino-acid analyses and ¹³C-NMR spectra of perchloric acid extracts of cells incubated for 4 h with [1-¹³C]glucose in the absence of extracellular glutamine. However, the resonances of a glutamate-linked product are observed in these spectra. The analysis of the pH dependence of chemical shifts from various glutamate-derived compounds shows that the observed resonances came from glutathione. Glutamine and glutathione signals are in close proximity on the frequency scale, leading to possible misinterpretation of the spectra.

【 授权许可】

Unknown