A novel property of a mitochondrial presequence

FEBS Letters

de Gier, Johannes¹  Leenhouts, Johanna M.¹  de Kruijff, Ben¹ 

[1] Centre for Biomembranes and Lipid Enzymology, Department of Biochemistry of Membranes, University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands

关键词: Mitochondrial presequence;    Membrane potential;    Membrane contact;    Cardiolipin;    ΔΨ;    membrane potential;    CL;    cardiolipin;    pCoxIV;    presequence of cytochrome oxidase subunit IV;    DOPS;    1;    2-dioleoyl-sn-glycero-3-phosphoserine;    DOPG;    1;    2-dioleoyl-sn-glycero-3-phosphoglycerol;    DOPA;    1;    2-dioleoyl-sn-glycero-3-phosphate;    DOPC;    1.2-dioleoyl-sn-glycero-3-phosphocholine;    PI;    phosphatidylinositol;    FCCP;    carbonylcyanide-p-trifuoromethoxy-phenylhydrazone;    LUVs;    large unilamellar vesicles;   

DOI  :  10.1016/0014-5793(93)80164-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A new property of the presequence of the mitochondrial precursor protein cytochrome oxidase subunit IV is presented. This mitochondrial presequence induces interbilayer contacts between large unilamellar vesicles consisting of phosphatidylcholine and cardiolipin. The presequence-vesicle aggregates can be dissociated by applying a membrane potential across the bilayers (negative inside). These effects require the presence of cardiolipin and are not observed for other negatively charged phospholipids. We propose a role for the presequence in the formation and dissociation of mitochondrial contact sites.

【 授权许可】

Unknown