Candida parapsilosis expresses and secretes two aspartic proteinases

FEBS Letters

Foundling, Stephen I.¹  Monod, Michel²  Fusek, Martin¹  Smith, Elizabeth A.¹ 

[1] Laboratory of Protein Crystallography, Protein Studies Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA;Laboratoire de Mycologie, Service de Dermatologie, Centre Hospitalier Universitaire Vaudois, 1011 Lausanne, Switzerland

关键词: Aspartic proteinase;    Proteinase inhibitor;    Pseudogene;    Candida parapsilosis;    SDS;    sodium dodecyl sulfate;    BSA;    bovine serum albumin;    CPAP;    Candida parapsilosis aspartic proteinase;   

DOI  :  10.1016/0014-5793(93)81050-A

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have isolated and characterized a second aspartic proteinase secreted by the CHUV E-18 strain of Candida parapsilosis. This proteinase is produced at a level corresponding to approximately 25% of the production of the main proteinase described earlier [1]. This minor proteinase has similar molecular weight and pH optimum but differs in the isoelectric point and in the specificity when compared with the major secreted form. The determination of the amino terminal amino acid sequence identified this minor form of Candida parapsilosis aspartic proteinase as a protein which corresponds to the sequence deduced from genomic DNA originally reported as a pseudogene [1]. We conclude that strain CHUV E-18 of Candida parapsilosis expresses and secretes two different aspartic proteinases.

【 授权许可】

Unknown