Negative growth control by a novel low M r phosphotyrosine protein phosphatase in normal and transformed cells

FEBS Letters

Raugei, Giovanni²  Rigacci, Stefania²  Ramponi, Giampietro²  Magnelli, Lucia³  Camici, Guido²  Ruggiero, Marco³  Pierce, Jacalyn H.¹  Pazzagli, Claudia²  Berti, Andrea²  Chiarugi, Vincenzo P.³ 

[1] LCMB, National Cancer Institute, NIH, Bethesda, 20892 MD, USA;Department of Biochemical Sciences, University of Firenze, viale Morgagni 50, 50134 Firenze, Italy;Institute of General Pathology, University of Firenze, viale Morgagni 50, 50134 Firenze, Italy

关键词: Phosphatase;    Oncogene;    Neoplasia;    Inositol lipid;   

DOI  :  10.1016/0014-5793(93)81811-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Having determined the complete amino acid sequence of a cytosolic phosphatase purified from bovine liver, we studied the role of this enzyme (referred to as ‘PTPase’) in the control of cell proliferation. We used NIH/3T3 fibroblasts, both normal and transformed by the oncogenes v-erbB, v-src, and v-raf: a synthetic gene coding for PTPase was transfected into, and overexpressed in, normal and transformed NIH/3T3 cells with resulting inhibition of cell growth. Inhibition of proliferation correlated with the level of foreign PTPase; growth in soft agar was also inhibited in transformants overexpressing the enzyme. However, PTPase overexpression did not inhibit the rapid turnover of inositol lipids stimulated by platelet-derived growth factor. We conclude that this novel PTPase is active on cell type-specific signalling substrates that control normal and transformed fibroblast proliferation.

【 授权许可】

Unknown