Immunopurification of a subcomplex of the NAD(P)H‐plastoquinone‐oxidoreductase from the cyanobacterium Synechocystis sp. PCC6803

FEBS Letters

Kinzelt, Dagmar²  Ellersiek, Ulrike¹  Steinmüller, Klaus¹  Berger, Susanne¹ 

[1] Institut für Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine-Universität, Universitätsstrasse 1, 4000 Düsseldorf 1, Germany;Institut für Enzymtechnologie, Forschungszentrum Jülich, Postfach 2050, 5170 Jülich 1, Germany

关键词: NAD(P)H-dehydrogenase;    Cyanobacteria;    Synechocystis sp. PCC6803;    NDH-H;    NDH-I;    NDH-J;    NDH-K;   

DOI  :  10.1016/0014-5793(93)81800-F

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An antibody against the NDH-K subunit of the NAD(P)H-dehydrogenase from the cyanobacterium Synechocystis sp. PCC6803 was used to isolate a subcomplex of the enzyme from Triton X-100 solubilized total membranes by immunoaffinity chromatography. The isolated subcomplex consisted of seven major polypeptides with molecular masses of 43, 27, 24, 21, 18, 14 and 7 kDa. The amino-terminal amino acid sequences of the polypeptides were determined. By comparing the sequences with the amino acid sequences deduced from DNA, three proteins were identified as NDH-H (43 kDa), NDH-K (27 kDa) and NDH-I (24 kDa). A fourth subunit (NDH-J, 21 kDa) was identified by Western blot analysis with an NDH-J antibody.

【 授权许可】

Unknown