Purification and sequence determination of heat‐stable enterotoxin elaborated by a cholera toxin‐producing strain of Vibrio cholerae O1

FEBS Letters

Miyachi, Miki²  Takeda, Tae¹  Xiaozhe, Huang¹  Nair, G.Balakrish³  Yoshino, Ken-ichi²  Shimonishi, Yasutsugu²  Takao, Toshifumi²  Bag, Prasanta K.³ 

[1] Department of Infectious Diseases Research, National Children's Medical Research Center, Taishido 3-35-31, Setagaya-ku, Tokyo 154, Japan;Institute for Protein Research, Osaka University, Yamadaoka 3-2, Suita, Osaka 565, Japan;National Institute of Cholera and Enteric Diseases, P-33, CIT Scheme XM, Beliaghata, Calcutta-700 010, India

关键词: Heat-stable enterotoxin;    Primary structure;    Cholera;    Vibrio cholerae O1;   

DOI  :  10.1016/0014-5793(93)81766-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Four molecular species of heat-stable enterotoxins elaborated by a cholera toxin-producing strain of Vibrio cholerae O1 were isolated from its culture supernatant. The amino acid sequence of one of the enterotoxins was determined to be Phe-Ile-Lys-Gln-Val-Asp-Glu-Asn-Gly-Asn-Leu-Ile-Asp-Cys-Cys-Glu-Ile-Cys-Cys-Asn-Pro-Ala-Cys-Phe-Gly-Cys-Leu-Asn with three intramolecular disulfide linkages. The other enterotoxins had shorter amino acid sequences in the N-terminal regions, but possessed the same sequence in their C-terminal regions including the three disulfide linkages. The enterotoxins with the shorter N-terminal sequences showed more potent toxicities, and the minimum effective dose of the longest one with 28 amino acid residues was 10-folds of that of the shortest one.

【 授权许可】

Unknown