FEBS Letters | |
Unique expression pattern of protein kinase C‐θ: high mRNA levels in normal mouse testes and in T‐lymphocytic cells and neoplasms | |
Osada, Shin-ichi2  Mushinsk, J.Frederic1  Ohno, Shigeo2  Henderson, Darren W.1  Mischak, Harald1  Goodnight, JoAnne1  | |
[1] Molecular Genetics Section, Laboratory of Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA;Department of Molecular Biology, Yokohama City University School of Medicine, 3-9, Fuku-ura, Kanazawa-ku, Yokohama 236, Japan | |
关键词: Protein kinase C; Isoenzyme; Expression; Lymphocyte; Testis; Lymphoma; Skeletal muscle; | |
DOI : 10.1016/0014-5793(93)81759-S | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
A 2.2-kb cDNA that contains the entire coding region of mouse protein kinase C-θ (PKC-θ) was cloned from skeletal muscle mRNA using reverse transcription and the polymerase chain reaction (PCR). This clone was used as a probe to study the expression of this PKC isoform in normal and transformed hemopoietic cells and other normal tissues. By far the highest steady-state level of PKC-0 MRNA was found as a 2.8-kb transcript on a Northern blot of poly(A)+ RNA from testes. High levels were also found in skeletal muscle, spleen, T lymphomas and purified normal T lymphocytes, but these tissues and cells expressed two transcripts, 3.3 kb and 3.8 kb. Lower levels of similar size transcripts were found in normal brain, B lymphocytes and B-lymphocytic tumors and cell lines.
【 授权许可】
Unknown
【 预 览 】
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RO201912020298080ZK.pdf | 1208KB | download |