Unique expression pattern of protein kinase C‐θ: high mRNA levels in normal mouse testes and in T‐lymphocytic cells and neoplasms

FEBS Letters

Osada, Shin-ichi²  Mushinsk, J.Frederic¹  Ohno, Shigeo²  Henderson, Darren W.¹  Mischak, Harald¹  Goodnight, JoAnne¹ 

[1] Molecular Genetics Section, Laboratory of Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA;Department of Molecular Biology, Yokohama City University School of Medicine, 3-9, Fuku-ura, Kanazawa-ku, Yokohama 236, Japan

关键词: Protein kinase C;    Isoenzyme;    Expression;    Lymphocyte;    Testis;    Lymphoma;    Skeletal muscle;   

DOI  :  10.1016/0014-5793(93)81759-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A 2.2-kb cDNA that contains the entire coding region of mouse protein kinase C-θ (PKC-θ) was cloned from skeletal muscle mRNA using reverse transcription and the polymerase chain reaction (PCR). This clone was used as a probe to study the expression of this PKC isoform in normal and transformed hemopoietic cells and other normal tissues. By far the highest steady-state level of PKC-0 MRNA was found as a 2.8-kb transcript on a Northern blot of poly(A)⁺ RNA from testes. High levels were also found in skeletal muscle, spleen, T lymphomas and purified normal T lymphocytes, but these tissues and cells expressed two transcripts, 3.3 kb and 3.8 kb. Lower levels of similar size transcripts were found in normal brain, B lymphocytes and B-lymphocytic tumors and cell lines.

【 授权许可】

Unknown