Location of tolerated insertions/deletions in the structure of the maltose binding protein

FEBS Letters

Saurin, William¹  Martineau, Pierre¹  Betton, Jean-Michel¹  Hofnung, Maurice¹ 

[1] Unité de Programmation Moléculaire et de Toxicologie Génétique (CNRS UA1444), Département des Biotechnologies, Institut Pasteur, Paris, France

关键词: Insertion/deletion;    Linker insertion mutagenesis;    Maltose binding protein;    Protein structure;   

DOI  :  10.1016/0014-5793(93)81409-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In a previous study [(1987) J. Mol. Biol. 194, 663-673], we isolated ten insertion/deletion mutants (indels) of the maltose binding protein for which the maltose binding constant was only a little or not at all affected. In this paper, we have localized these mutations in the recently solved three-dimensional structure. Contrary to the general expectation, most of the insertion/deletion modifications occurred within elements of secondary structure. An analysis of the inserted residues for three indels found within α helices allowed an interpretation regarding protein structure accommodation to such modifications.

【 授权许可】

Unknown