Detection of a 10 kDa breakdown product containing the C‐terminus of the D 1‐protein in photoinhibited wheat leaves suggests an acceptor side mechanism

FEBS Letters

Cánovas, Pedro M.¹  Barber, James¹ 

[1] AFRC Photosynthesis Research Group, Wolfson Laboratories, Biochemistry Department, Imperial College of Science, Technology and Medicine, London SW7 2AY, UK

关键词: Photo-inhibition;    Photosystem II;    D1-protein;    PAGE;    polyacrylamide gel electrophoresis;    PSII;    photosystem II;    SDS;    sodium dodecyl sulphate;   

DOI  :  10.1016/0014-5793(93)80147-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Photoinhibition of intact leaves of wheat generates a 10 kDa breakdown product which is clearly observed both at 4°C and 25°C. Selective immunoblotting has shown that the 10 kDa fragment contains the C-terminus of the D 1-protein and, under the conditions employed, supports an acceptor side mechanism for photoinhibition in vivo. Although a corresponding 23 kDa N-terminal Dl-fragment was not detected our results are consistent with the argument that the primary cleavage site is in the loop joining putative transmembrane segments IV and V.

【 授权许可】

Unknown