A tandem repeat of the SPKK peptide motif induces Ψ‐type DNA structures at alternating AT sequences

FEBS Letters

Colson, Pierre²  Bailly, Fabrice³  Houssier, Claude²  Bailly, Christian³  Hénichart, Jean-Pierre¹ 

[1] UCB Pharmaceuticals, Chemin du Foriest, B-1420 Braine-l'Alleud, Belgium;Laboratoire de Chimie Macromoléculaire et Chimie Physique, Université de Liège, Sart-Tilman, B-4000 Liège, Belgium;INSERM unité 124, Institut de Recherches sur le Cancer, Place de Verdun, 59045 Lille, France

关键词: DNA condensation;    Histone;    Circular dichroism;    DNA sequence-specificity;    Basic peptide;    Transcription;    CD;    circular dichroism;    CT;    calf thymus;    SPKK;    Ser-Pro-Lys-Lys;    R;    Arg;    T;    Thr;   

DOI  :  10.1016/0014-5793(93)81389-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The interaction between a tandem repeat of the SPKK peptide motif and calf thymus DNA or several polynucleotides has been investigated by circular dichroism. The octapeptide SPKKSPKK does not induce any important changes in the CD spectra of the polynucleotides poly(dG) · poly(dC), poly(dG-dC) · poly(dG-dC) and poly(dA) · poly(dT) while the spectrum of calf thymus DNA is slightly modified. Binding of this basic peptide to the alternating copolymer poly(dA-dT) · poly(dA-dT) results in a marked Ψ-type condensation in a manner similar to that induced by the entire C-terminal domain of histone H1.

【 授权许可】

Unknown