Application of poly(A)+RNA patterns method for searching of differentially expressed genes

FEBS Letters

Diachenko, Ludmila B.¹  Chenchik, Alexander A.¹  Beabealashvilli, Robert Sh.¹ 

[1] Cardiology Research Center, Russian Academy of Medical Sciences, 3rd Cherepkovskaya 15A, Moscow, 121552, Russian Federation

关键词: Poly(A)+RNA pattern;    Reverse transcriptase;    Differentially expressed genes;    Fetal liver;    Cirrhosis;    F9 embryonal carcinoma;   

DOI  :  10.1016/0014-5793(93)81379-E

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Poly(A)⁺RNA composition differences for normal, fetal and cirrhotic human liver before and after retinoic acid-induced differentiation of the F9 embryonal carcinoma cell line were analyzed by a novel poly(A)⁺RNA patterns method. The method is based on the polyacrylamide gel electrophoretic analysis of short cDNA termination products, synthesized by reverse transcriptase using poly(A)⁺RNA as a template, a set of short 5'-end labeled primers, three natural and one terminator deoxyribonucleotide. A number of known differentially expressed genes and some unknown ones were then identified by direct sequencing of the differentially represented bands excised from a gel and searching a complementary mRNA target sites in GenBank database.

【 授权许可】

Unknown