Calvasculin, as a factor affecting the microfilament assemblies in rat fibroblasts transfected by src gene

FEBS Letters

Usada, Nobuteru¹  Tsugane, Shinichiro¹  Ishikawa, Ryoki¹  Kohama, Kazuhiro¹  Morita, Takashi¹  Watanabe, Yasuo¹  Hidaka, Hiroyoshi¹  Minami, Hiroyuki¹  Tomida, Yasuhiro¹ 

[1] Department of Pharmacology, Nagoya University School of Medicine, Tsuramai 65, Showa-ku, Nagoya 466, Japan

关键词: Calvasculin;    EF-hand;    F-actin;    Microfilament;    Rat fibroblasts transfected by src gene;    SR-3Y1 cell;    EGTA;    ethylenebis(oxyethylenenitrilo) tetraacetic acid;    Tris;    2-amino-2-(hydroxymethyl)-1;    3-propanediol;    PBS;    phosphate-buffered saline;   

DOI  :  10.1016/0014-5793(93)81530-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cell transformations accompany alterations in cell morphology and microfilament patterns. Calvasculin encodes mRNA termed pEL-98, 18A2, 42A, p9Ka, or mtsl, found to be elevated in several metastatic cell lines. We report the elevation of calvasculin expression in SR-3Y1 cells, which show disappearance of ordered microfilaments, compared to that in 3Y1 cells and that the similar distribution of calvasculin to that of actin filaments. Interestingly, calvasculin co-sediments with F-actin and bundles actin filaments in a Ca²⁺-dependent manner. This activity, along with the elevation of calvasculin following transformation, suggests that the disorganization of filaments in SR-3Y1 cell is due to the cross-linking activity of calvasculin.

【 授权许可】

Unknown