Presence of the gypsy (MDG4) retrotransposon in extracellular virus‐like particles

FEBS Letters

Semakin, Andrej B.²  Tsuprun, Vladimir L.³  Stepanov, Alexander S.¹  Kandror, Konstantin V.¹  Syomin, Boris V.² 

[1] Bakh Institute of Biochemistry, Russian Academy of Sciences, Moscow, 117071, Russian Federation;Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 117984, Russian Federation;Shubnikov Institute of Crystallography, Russian Academy of Sciences, Moscow, 117033, Russian Federation

关键词: Retrotransposon;    Virus-like particle;    gypsy;   

DOI  :  10.1016/0014-5793(93)81358-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

As a first step to investigate the functional activity of gypsy virus-like particles (VLPs) we explored the possibility of preservation of its VLP in extracellular form. The preparations containing extracellular gypsy VLP from Drosophila melanogaster and D. virilis were obtained. Full-length polyA⁺ RNA and polyA⁺ RNA-DNA complexes of gypsy were observed in both preparations. The polypeptides with some specificity to gypsy nucleic acids were identified in the obtained VLP preparations. These data accompanied by morphological characteristics of samples testify the presence of intact gypsy VLP in cultured media both from D. melanogaster and D. virilis cultivated cells.

【 授权许可】

Unknown