Calcium‐induced degradation of the Inositol (1,4,5)‐trisphosphate receptor/Ca2+‐channel

FEBS Letters

Østvold, Anne Carme¹  Walaas, S.Ivar¹  Magnusson, Andres¹  Haug, Lise Sofie¹ 

[1] Neurochemical Laboratory, University of Oslo, POB 1115, Blindern 0317, Oslo 3, Norway

关键词: Calpain;    Ca2+-channel;    IP3-receptor;    Signal transduction;    Cerebellum;   

DOI  :  10.1016/0014-5793(93)81345-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Ca²⁺-induced degradation of the neuronal inositol (1,4,5)-trisphosphate receptor, a protein which regulates Ca²⁺-release from intracellular stores, has been examined. The IP₃-receptor, immunopurified from rat cerebellum, appeared to be an excellent substrate for purified Ca²⁺-activated neutral protease (calpain). Incubation of membranes or immunopurified IP₃-receptor with Ca²⁺ and cerebellar cytosol also resulted in degradation of the receptor. Two main fragments with approximate molecular masses of 130 and 95 kDa were generated, both of which appeared to derive from the carboxyterminal Ca²⁺-channel-containing part of the protein. These data suggest that activation of the IP₃-receptor, by causing increases in intracellular [Ca²⁺], might result in degradation of the N-terminal, IP₃-binding part of the receptor.

【 授权许可】

Unknown