High level expression of the large subunit of mouse ribonucleotide reductase in a baculovirus system

FEBS Letters

Cooperman, Barry S.¹  Scott, Charles P.¹  Li, Lin-Sheng²  Rubin, Harvey²  Salem, Jerome S.² 

[1] Department of Chemistry, The University of Pennsylvania, 34th and Spruce St., Philadelphia, PA 19104, USA;Department of Medicine, Infectious Diseases Section, 36th and Hamilton Walk, The University of Pennsylvania, Philadelphia, PA 19104, USA

关键词: Ribonucleotide reductase;    Expression;    Baculovirus;   

DOI  :  10.1016/0014-5793(93)81455-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The large subunit of ribonucleotide reductase from mouse has been overexpressed in Spodoptera frugiperda cells infected with recombinant baculovirus. The expressed protein was purified by affinity chromatography to apparent homogeneity as determined by SDS-PAGE. The homogeneous protein is recognized in Western blot analysis by a monoclonal antibody raised to the large subunit of ribonucleotide reductase from calf thymus, has the correct N-terminal sequence, and, in the presence of the small subunit of mouse ribonucleotide reductase and nucleoside triphosphate effectors, catalyzes the reduction of both purine and pyrimidine nucleoside diphosphates.

【 授权许可】

Unknown