Isolation, characterization and partial sequencing of Pregnancy Associated Mouse Protein PAMP1 identifies it as a novel female specific protein, unrelated to the α‐2‐macroglobulin family of proteinase inhibitors

FEBS Letters

Torrekens, Sophie¹  Van den Berghe, Herman¹  Van Leuven, Fred¹ 

[1] Experimental Genetics Group, Center for Human Genetics, Katholieke Universiteit van Leuven, Campus Gasthuisberg O&N 6, B-3000 Leuven, Belgium

关键词: Pregnancy protein;    α-2-Macroglobulin;    A2M;    α-2-macroglobulin;    PZP;    pregnancy zone protein;    PAMP;    pregnancy associated mouse protein;    SDS;    sodium dodecyl sulfate;    PBS;    phosphate-buffered saline;    TBS;    Tris-buffered saline;   

DOI  :  10.1016/0014-5793(93)81573-I

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Pregnancy Associated Mouse Protein 1 (PAMP1) was isolated from plasma of female mice. An antiserum raised against the purified protein confirmed its immunochemical identity with the originally described PAMP1. Pregnant females were observed to have plasma levels of PAMP1 that are increased two-fold at day 10–13 of gestation relative to non-pregnant females, while male mouse plasma did not contain PAMP1. The purified protein displayed an apparent subunit molecular mass of 70 kDa, irrespective of cystine reduction. The native molecular mass, estimated by gel-filtration, was about 140 kDa, indicating that PAMP1 is circulating as a non-covalent homodimer. The amino-terminal sequence of the intact protein and the internal sequences of four cyanogen bromide fragments demonstrated that this protein is not related to any known member of the α-2-macroglobulin family nor to any protein in the sequence databases. The physicochemical and the sequence data thus establish this protein as a novel, female-specific protein, but unrelated to the Macroglobulin proteinase inhibitor family.

【 授权许可】

Unknown