Monoclonal anti‐biotin antibodies simulate avidin m the recognition of biotin

FEBS Letters

Bagçi, Hasan¹  Kohen, Fortune¹  Bayer, Edward A.²  Kusçuoglu, Unsal¹  Wilchek, Meir² 

[1] Department of Hormone Research, The Weizmann Institute of Science, Rehovot 76100, Israel;Department of Biophysics, The Weizmann Institute of Science, Rehovot 76100, Israel

关键词: Anti-biotin antibody;    Antibody gene;    Biotin binding site;    Antibody combining site;    Binding motif;   

DOI  :  10.1016/0014-5793(93)81108-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The sequence of the V_H gene of a monoclonal anti-biotin antibody was determined. Biotin-binding motifs, similar to those in avidin and streptavidin, were identified in complementarity determining regions 2 and 3, suggesting that natural selection of functional motifs may occur in unrelated protein types.

【 授权许可】

Unknown