A human T lymphotropic virus type I (HTLV‐I) long terminal repeat‐directed antisense c‐myc construct with an Epstein‐Barr virus replicon vector inhibits cell growth in a HTLV‐I‐transformed human T cell line

FEBS Letters

Fujita, Masatoshi¹  Shiku, Hiroshi¹ 

[1] Department of Oncology, Nagasaki University School of Medicine, 1-12-4 Sakamoto, Nagasaki 852, Japan

关键词: HTLV-I;    EBV replicon vector;    Gene transfection;    Antisense RNA;    c-myc;    HTLV-I;    human T lymphotropic virus type I;    ATL;    adult T cell leukemia;    EBV;    Epstein-Barr virus;    RSV;    Rous sarcoma virus;    CMV;    cytomegalovirus;    LTR;    long terminal repeat;    CAT;    chloramphenicol acetyltransferase;    FCS;    fetal calf serum;    PBS;    phosphate-buffered saline;    mAb;    monoclonal antibody;    ECL;    enhanced chemiluminescence;   

DOI  :  10.1016/0014-5793(93)81101-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A panel of EB virus replicon-based vectors was constructed to examine the relative utility of four distinct eukaryotic promoters for high-level gene expression in a HTLV-I-transformed human T cell line, HUT102. We found that HTLV-I LTR, which is trans-activated by the viral tax protein, was most suited for EBV vector-based stable gene expression in it. We prepared a HTLV-I LTR-directed antisense c-myc construct with an EBV vector. This antisense plasmid suppressed c-myc expression and inhibited growth of HUT102 cells in vitro with unaltered expression of tax. Non-specific plasmid toxicity was excluded by showing that the antisense construct had little effect on growth and c-myc expression of HTLV-I-negative Jurkat T cells, in which the viral LTR is expected to be less active. Our results indicate that c-myc may play an important role in the deregulated growth of HTLV-I-transformed T cells.

【 授权许可】

Unknown