Participation of cathepsin L on bone resorption

FEBS Letters

Kakegawa, Hisao¹  Kamioka, Hiroshi³  Katunuma, Nobuhiko¹  Lenarčič, Brigita⁴  Sumitani, Koji³  Kawata, Terushige³  Nikawa, Takeshi²  Drobnič-Kosorok, Marinka⁴  Tagami, Kahori³  Turk, Vito⁴ 

[1]Institute for Health Sciences, Tokushima Bunri University, Yamashiro-cho, Tokushima 770, Japan

[2]Division of Enzyme Chemistry, Institute for Enzyme Research, The University of Tokushima, Kuramoto-cho, Tokushima 770, Japan

[3]Department of Orthodontics, School of Dentistry, The University of Tokushima, Kuramoto-cho, Tokushima 770, Japan

[4]Department of Biochemistry, J. Stefan Institute, Jamova, 39, 61000 Ljubljana, Slovenia

关键词: Cathepsin L;    E-64;    CA-074;    Cystatin A;    Pig leucocyte cysteine proteinase inhibitor;    Bone resorption;    Z;    benzyloxycarbonyl;    MCA;    methylcoumarylamide;    BCA;    bicinchoninic acid;    OCPC;    o-cresolphthalein complexone;    E-64-a;    N-(l-3-trans-carboxyoxirane-2-carbonyl)-l-leucine-4-aminobutylamide;    CA-074;    N-(l-3-trans-propylcarbamoyloxirane-2-carbonyl)-l-isoleucyl-l-proline. Enzymes: cathepsin B;    EC 3.4.22.1;    cathepsin L;    EC 3.4.22.15;   

DOI  :  10.1016/0014-5793(93)80118-E

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The proteinase responsible for bone collagen degradation in osteo-resorption was examined. The bone pit formation induced by parathyroid hormone (PTH) was markedly suppressed by leupeptin, E-64 and cystatin A, while no inhibition was observed by CA-074, a specific inhibitor of cathepsin B. Pig leucocyte cysteine proteinase inhibitor (PLCPI), a specific inhibitor of cathepsin L, and chymostatin, a selective inhibitor of cathepsin L, completely inhibited the pit formation. Cathepsin L activity in osteoclasts was much higher than the other cathepsin activities. Serum calcium in rats placed on a low calcium diet was decreased by treatment of E-64 or cystatin A, but not by CA-074. These findings suggest that cathepsin L is the main proteinase responsible for bone collagen degradation.

【 授权许可】

Unknown