Insight into topological and functional relationships of cytochrome c oxidase subunit I of Saccharomyces cerevisiae by means of intragenic complementation

FEBS Letters

Lemarre, Philippe¹  Colson, Anne-Marie¹  Coster, Françoise¹  Meunier, Brigitte¹ 

[1] Université Catholique de Louvain, Laboratoire de Génétique Microbienne, Place Croix du Sud 4, 1348 Louvain-la-Neuve, Belgium

关键词: Cytochrome c oxidase;    Yeast;    Intragenic complementation;    Models;   

DOI  :  10.1016/0014-5793(93)80099-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In yeast, revertants were selected from four respiratory deficient mutants carrying mutations in the cytochrome c oxidase subunit I gene. Intragenic second site mutations revealed amino acids which are functionally complementary to the original mutated position and may be in topological interaction with it. The results provide additional data in favour of the model proposed for the structure of the binuclear centre in proton-motive oxidases.

【 授权许可】

Unknown