Bovine inositol monophosphatase

FEBS Letters

Gore, M.G.²  Greasley, P.J.²  Ragan, C.I.¹  Rees-Milton, K.J.² 

[1] Neurosciences Research Centre, Merck Sharp and Dohme, Harlow, Essex, UK;SERC Centre for Molecular Recognition, Department of Biochemistry, University of Southampton, Southampton, S09 3TU, UK

关键词: Inositol monophosphatase;    Histidine;    Diethylpyrocarbonate;    Site-directed mutagenesis;    DEP;    diethylpyrocarbonate;    IMPase;    inositol monophosphatase;   

DOI  :  10.1016/0014-5793(93)80616-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The inositol monophosphatase from bovine brain is inactivated by the histidine-specific reagent diethylpyrocarbonate. Using 4 mM reagent at pH 6.5, the reaction results in the modification of 3 equivalents of histidine per polypeptide chain. The loss of activity occurs at the same rate as the slowest reacting of these residues. Site directed mutagenesis studies have been used to generate a mutated enzyme species bearing a His-217→Gln replacement and have shown that it is the modification of histidine 217 which results in the inactivation of the enzyme.

【 授权许可】

Unknown