Ca2+ binding of latent transforming growth factor‐β1 binding protein

FEBS Letters

Hellman, Ulf¹  Heldin, Carl-Henrik¹  Colosetti, Pascal¹  Miyazono, Kohei¹ 

[1] Ludwig Institute/or Cancer Research, Box 595, Biomedical Center, S-751 24 Uppsala, Sweden

关键词: Transforming growth factorβ;    Binding protein;    EGF-like repeat;    Ca2+ binding;    TGF-β;    transforming growth factor-β;    LTBP;    latent TGF-βl binding protein;    LL-TGF-β1;    large latent TGF-β1 complex;    β1-LAP;    TGF-β1 latency associated peptide;    EGF;    epidermal growth factor;   

DOI  :  10.1016/0014-5793(93)80079-A

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Latent transforming growth factor-β1 binding protein (LTBP) is a constituent of the latent high molecular weight complex of TGF-β1 in human platelets. In the present communication, we show that LTBP binds Ca²⁺ in its free form as well as in the latent TGF-β1 complex. The binding of Ca²⁺ induces a structural change which protects the molecule against proteolysis and changes its elution position when analyzed by anion exchange chromatography. The in vitro activation of TGF-β1 is not influenced by the presence of Ca²⁺. The possible significance of Ca²⁺ binding of LTBP is discussed.

【 授权许可】

Unknown