Amino acid sequence of alkaliphilic serine protease from silkworm, Bombyx mori, larval digestive juice

FEBS Letters

Ichikawa, Katsuomi¹  Sasaki, Takuji¹  Hishida, Tomoaki¹  Asari, Shin-ichiro¹ 

[1] Department of Food Science and Technology, School of Agriculture, Nagoya University, Chikusa-ku, Nagoya, Aichi 464-01, Japan

关键词: Amino acid sequence;    Trypsin-like protease;    Alkaliphilic serine protease;    Silkworm larva;    Bombyx mori;   

DOI  :  10.1016/0014-5793(93)81652-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Alkaliphilic protease, P-IIc, from silkworm, Bombyx mori, larval midgut digestive juice consists of 232 amino acids. It has a catalytic triad, Asp-His-Ser, invariably found in a serine protease. A shift of optimal pH value towards the alkaline side diminished at μ=1.0. This suggests the existence of an electrostatic interaction that affects the proteolytic activity. The higher Arg content may be responsible for this phenomenon. Two cysteine residues probably exist unpaired in a novel position among serine proteases.

【 授权许可】

Unknown