FEBS Letters | |
A pH induced two‐dimensional crystal of membrane‐bound Na+,K+‐ATPase of dog kidney | |
Fujiyoshi, Yoshinori1  Tahara, Yoshikazu3  Kimura, Yoshiaki1  Ohnishi, Shun-ichi3  Hayashi, Yutaro2  | |
[1] Protein Engineering Research Institute, 6-2-3 Furuedai, Suita, Osaka 565, Japan;Department Biochemistry, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka, Tokyo 181, Japan;Department of Biophysics, Faculty of Science, Kyoto University, Sakyo-ku, Kyoto 606, Japan | |
关键词: Sodium potassium adenosine triphosphatase (Na+; K+-ATPase); Two-dimensional crystallization; Ice-embedding technique; Electron cryo-microscopy; Computer assisted image processing; | |
DOI : 10.1016/0014-5793(93)81648-J | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Two-dimensional crystals of membrane-bound Na+,K+-ATPase were formed in acidic media and their qualities were investigated by electron cryo-microscopy as well as by conventional electron microscopy. At pH 4.8 in sodium citrate buffer, the best crystallization condition, more than 80% of membranes formed crystals. The high ratio allowed high-resolution images to be taken by electron cryo-microscopy. Image processing revealed that they had unique lattice constants (a = 108.7 Å, b = 66.2 Å, γ = 104.2°) and had few defects in the crystalline arrays. The reconstituted Fourier map of the ice-embedded crystal showed that there are two high contrast parts in one unit cell.
【 授权许可】
Unknown
【 预 览 】
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