Activation of adenylate cyclase in cdc25 mutants of Saccharomyces cerevisiae

FEBS Letters

Lazo, Pedro S.¹  Ramos, Sofia¹  Pardo, Luis A.¹ 

[1]Departamento de Biología Funcional, Universidad de Oviedo, E-33071 Oviedo, Spain

关键词: Adenylate cyclase;    Ras protein;    Cdc25;    Glucose;    Yeast;    6dG;    6-deoxy-d-glucose;    GDPßS;    guanosine 5'-O-(2-thyodiphosphate);    GppNHp;    guanylyl imidodiphosphate;    MES;    2-(N-morpholino)ethanesulfonic acid;   

DOI  :  10.1016/0014-5793(93)80554-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The activation of adenylate cyclase by guanine nucleotides and 6-deoxyglucose was studied in membrane preparations from S. cerevisiae mutants lacking the CDC25 gene product. Adenylate cyclase from cdc25 ts membranes was activated by GTP and GppNHp in membranes from cells collected after glucose was exhausted from the medium. The activation was also observed in membranes from repressed cells at 2.5 mM Mg²⁺. It is also shown that 6-deoxyglucose can activate adenylate cyclase in the absence of CDC25 gene product. The relative amount of membrane-bound adenylate cyclase was drastically reduced in cdc25 ts membranes when subjected to the restrictive temperature, while no significant change was observed in the wild type. These data suggest that Cdc25 might not be required in certain conditions for the guanine nucleotide exchange reaction in Ras and that it might be implicated in anchoring the Ras/adenylate cyclase system to the plasma membrane.

【 授权许可】

Unknown