Cloning and sequencing of glutamate mutase component E from Clostridium tetanomorphum

FEBS Letters

Kellermann, J.¹  Brecht, M.³  Pluckthun, A.² 

[1] Max-Planck-Institut für Biochemie, Proteinchemie, Protein Engineering, Am Klopferspitz 18a, 8033 Martinsried, Germany;Max-Planck-Institut für Biochemie, Protein Engineering, Am Klopferspitz 18a, 8033 Martinsried, Germany;Genzentrum der Universität München, Protein Engineering, Am Klopferspitz 18a, 8033 Martinsried, Germany

关键词: Glutamate mutase;    Cobalamin-dependent enzyme;    Vitamin B12;   

DOI  :  10.1016/0014-5793(93)80042-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The nucleotide sequence of the large subunit E of glutamate mutase of Clostridium tetanomorphum was determined. The protein consists of 483 amino acids and is not made in a precursor form, thus excluding the possibility of subunit E being a pyruvoyl enzyme. It shows no homology to any other protein in the database, and while binding coenzyme B₂, a conspicuous B₁₂ binding motif, shared amongst other proteins, is not detectable at the sequence level.

【 授权许可】

Unknown