A protein histidine kinase induced m rat liver by peroxisome proliferators

FEBS Letters

Goto, Sataro¹  Motojima, Kiyoto¹ 

[1] Department of Biochemistry, School of Pharmaceutical Sciences, Toho University, Funabashi, Chiba 274, Japan

关键词: Histidine kinase;    Peroxisome proliferator;    Ras protein;    Signal transduction;    Rat liver;    P36;    a 36 kDa protein from rat liver;    PMS;    post-mito-chondrial supernatant;    PM;    plasma membrane;    PIPES;    piperazine-N;    N'-bis(2-ethanesulfonic acid);    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;    TLC;    thin layer chromatography;    EGF;    epidermal growth factor;   

DOI  :  10.1016/0014-5793(93)80040-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A novel protein kinase is induced in rat liver plasma membrane by the administration of peroxisome proliferators. A 36 kDa protein (P36) on the membrane was rapidly phosphorylated in vitro by the kinase and the phosphorylated amino acid was identified as phosphohistidine. Histidine phosphorylation of P36 was activated in vitro by recombinant Ras protein and GTP; both decreased Michaelis constant (K _m) for ATP from 1.25 to 0.25 μM. The novel histidine kinase, products of which have been overlooked due to their acid lability, may participate in cellular signaling and peroxisome proliferators may perturb the pathway.

【 授权许可】

Unknown