Evidence for a membrane skeleton in higher plants

FEBS Letters

Spanswick, Roger M.¹  Faraday, Christopher D.¹ 

[1] Section of Plant Biology, Division of Biological Sciences, Cornell University, Ithaca, NY 14853, USA

关键词: Aqueous polymer two-phase partitioning;    Fodrin;    Peripheral protein;    Cytoskeleton-plasma membrane interaction;    BSA;    bovine serum albumin;    BTP;    1;    3-bis[tris(hydroxymethyl)-methylamino]propane;    DTT;    dithiothreitol;    MES;    2-[N-morpholino]ethanesulfonic acid;    PMSF;    phenylmethylsulphonyl fluoride;    SDS-PAGE;    sodium dodecyl sulfate polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(93)80536-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A fraction enriched in plasma membranes was isolated from rice roots by differential centrifugation and aqueous polymer two-phase partitioning. Analysis of the fraction by SDS-PAGE showed the presence of several low mobility polypeptides (M _r> 100 kDa). One of these polypeptides (M _r ~ 230 kDa) was specifically recognized by polyclonal antibodies to human erythrocyte spectrin. This finding suggests that a higher plant spectrin-based membrane skeleton may be preserved and studied using high-purity plasma membrane fractions obtained by aqueous polymer two-phase partitioning.

【 授权许可】

Unknown