FEBS Letters | |
Carbohydrate structures of the cell adhesion molecule, contact site A, from Dictyostelium discoideum | |
Ouchi, Seiji3  Fuse, Goro3  Matsui, Taei1  Yoshida, Motonobu2  | |
[1] Institute for Comprehensive Medical Science, Fujita-Gakuen Health University, Toyoake, Aichi 470-11, Japan;Research Institute of Food Science, Kinki University, Higashi-Osaka, Osaka 577, Japan;Faculty of Agriculture, Kinki University, Higashi-Osaka, Osaka 577, Japan | |
关键词: Cell-to-cell interaction; Cell adhesion molecule; Carbohydrate recognition; Carbohydrate structure; Dictyostelium discoideum; | |
DOI : 10.1016/0014-5793(93)80534-2 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
We determined the carbohydrate structures of contact site A from Dictyostelium discoideum. The carbohydrate moieties of contact site A were released by hydrazinolysis. Fractionation of the deacidified oligosaccharide mixture by Bio-Gel P-4 column chromatography revealed that it was composed of four major oligosaccharides. Their respective structures were determined by sequential exoglycosidase digestion. It is known that contact site A consists of two kinds of carbohydrates, I and II. Taking together the previous and the present results, it was deduced that carbohydrate I comprises N-linked oligosaccharides and carbohydrate II O-linked ones. Furthermore, the relative molar contents of GalNAc and GlcNAc in reducing terminal suggested that contact site A contains 67% of N-linked and 33% of O-linked oligosaccharides.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020297547ZK.pdf | 532KB | download |