Carbohydrate structures of the cell adhesion molecule, contact site A, from Dictyostelium discoideum

FEBS Letters

Ouchi, Seiji³  Fuse, Goro³  Matsui, Taei¹  Yoshida, Motonobu² 

[1]Institute for Comprehensive Medical Science, Fujita-Gakuen Health University, Toyoake, Aichi 470-11, Japan

[2]Research Institute of Food Science, Kinki University, Higashi-Osaka, Osaka 577, Japan

[3]Faculty of Agriculture, Kinki University, Higashi-Osaka, Osaka 577, Japan

关键词: Cell-to-cell interaction;    Cell adhesion molecule;    Carbohydrate recognition;    Carbohydrate structure;    Dictyostelium discoideum;   

DOI  :  10.1016/0014-5793(93)80534-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We determined the carbohydrate structures of contact site A from Dictyostelium discoideum. The carbohydrate moieties of contact site A were released by hydrazinolysis. Fractionation of the deacidified oligosaccharide mixture by Bio-Gel P-4 column chromatography revealed that it was composed of four major oligosaccharides. Their respective structures were determined by sequential exoglycosidase digestion. It is known that contact site A consists of two kinds of carbohydrates, I and II. Taking together the previous and the present results, it was deduced that carbohydrate I comprises N-linked oligosaccharides and carbohydrate II O-linked ones. Furthermore, the relative molar contents of GalNAc and GlcNAc in reducing terminal suggested that contact site A contains 67% of N-linked and 33% of O-linked oligosaccharides.

【 授权许可】

Unknown