Reduction in the amount of 8‐hydroxy‐2'‐deoxyguanosine in the DNA of SV40‐transformed human fibroblasts as compared with normal cells in culture

FEBS Letters

Barciszewski, Jan¹  Clark, Brian F.C.²  Rattan, Suresh I.S.²  Otzen, Daniel E.²  Siboska, Gunhild E.² 

[1] Institute of Bioorganic Chemistry of the Polish Academy of Sciences, Noskowskiego 12, 61704 Poznan, Poland;Laboratory of Cellular Ageing, Department of Chemistry, Aarhus University, DK-8000 Aarhus-C, Denmark

关键词: Oxidative DNA damage;    8-Hydroxydeoxyguanosine;    Transformed cell;    HPLC/EC;   

DOI  :  10.1016/0014-5793(93)80018-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

DNA damage due to oxidative free radicals is considered to be a major cause of ageing and age-related diseases including cancer. Of more than 20 modifications formed in DNA by the action of hydroxyl radicals, 8-hydroxy-2'-deoxyguanosine (oh⁸dG) is potentially highly mutagenic and is known to occur most frequently. Using HPLC combined with electrochemical (HPLC/EC) detection of oh⁸dG, fivefold higher levels of oh⁸dG are detected in the DNA of cultured normal human skin fibroblasts as compared with SV40-transformed human fibroblasts MRC-5V2. In comparison, the levels of oh⁸dG were similar in the growth medium of both types of cells. Applications of this method range from studies on the genomic stability and instability of normal and cancerous cells to the clinical and laboratory testing of toxic substances and drugs.

【 授权许可】

Unknown