Tissue specificity of the regulation of ATP hydrolysis by isolated plant mitochondria

FEBS Letters

Diolez, Philippe²  Gardeström, Per¹  Valeric, Marie²  Haraux, Francis² 

[1] Department of Plant Physiology, University of Umeå, S-90187 Umeå, Sweden;Biosystèmes Membranaires (UPR 39), CNRS, Gif-sur-Yvette, France

关键词: Plant mitochondria;    F0F1 H+-ATPase;    Membrane potential;    ΔpH;    ATP-hydrolysis;    F0 and F1;    membranous and extrinsic parts of the proton ATPase;    Δg̃mH+ electrochemical proton gradient;    Δψ;    trans-membrane potential difference;    ΔpH;    transmembrane pH difference;    TPP+;    tetraphenylphosphonium ion;   

DOI  :  10.1016/0014-5793(93)80003-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Pea leaf mitochondria had a high ATP hydrolase activity following the collapse of the membrane potential by addition of valinomycin in state 4. In mitochondria isolated from potato tubers such ATP hydrolase activity was not observed. Pea leaf mitochondria also had a ΔpH, in contrast to what was previously found for potato tuber mitochondria. This ΔpH could, however, not explain the different results on ATP hydrolysis since this activity was also observed in the presence of nigericin. The results suggest a tissue-specific regulation of ATP hydrolysis in resting organs (potato tubers) as compared to active organs (leaves).

【 授权许可】

Unknown