Evidence for an [Fe]‐type hydrogenase in the parasitic protozoan Trichomonas vaginalis

FEBS Letters

Payne, Martin J.¹  Chapman, Alan²  Cammack, Richard¹ 

[1] Division of Life Sciences, King's College London, Campden Hill Road, London, W8 7AH, UK;The Wellcome Research Laboratories, Langley Court, Beckenham, Kent, BR3 3BS, UK

关键词: Pathogenic protozoa;    EPR spectroscopy;    Hydrogenase;    Iron-sulfur protein;    Trichomonas vaginalis;    C.;    Clostridium;    Chl.;    Chlamydomonas;    D.;    Desulfovibrio;    T.;    Trichomonas;    Tr.;    Tritrichomonas;    EPR;    electron paramagnetic resonance;   

DOI  :  10.1016/0014-5793(93)81500-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The hydrogenase of the pathogenic protozoan Trichomonas vaginalis was extracted and partially purified. The catalytic and spectroscopic properties of the enzyme indicate that it belongs to the class of [Fe]-hydrogenases, rather than the [NiFe]-hydrogenases. The hydrogenase activity was highly sensitive to carbon monoxide, 50% inhibition being attained by 1 μM CO. The EPR spectrum of the most active fractions from chromatography, after reduction by hydrogen and partial reoxidation under argon, showed an EPR spectrum at g = 2.10, 2.04, 2.00. This unusual spectrum is characteristic of the ‘H-cluster’, as seen in [Fe]-hydrogenases of anaerobic bacteria such as Clostridium spp.

【 授权许可】

Unknown