Two 3',5'‐cyclic‐adenosine monophosphate response elements in the promoter region of the human gastric inhibitory polypeptide gene

FEBS Letters

Someya, Yoshimichi⁴  Seino, Yutaka³  Ishii, Shunsuke¹  Inagaki, Nobuya²  Maekawa, Toshio¹ 

[1] Second Division, Laboratory of Molecular Genetics, Tsukuba Life Science Center, The Institute of Physical and Chemical Research (RIKEN), Tsukuba, Ibaraki 305, Japan;Second Division, Division of Molecular Medicine, Center of Neurobiology and Molecular Immunology, Chiba University School of Medicine, Chiba 280, Japan;Second Division, Department of Metabolism and Clinical Nutrition, Kyoto University School of Medicine, Kyoto 606, Japan;Second Division, Department of Medicine , Kyoto University School of Medicine, Kyoto 606, Japan

关键词: Gastric inhibitory protein;    cAMP response element;    Glucose;    Jun;   

DOI  :  10.1016/0014-5793(93)81493-J

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Transfection of chimeric chloramphenicol acetyltransferase plasmids containing various deletions of the human gastric inhibitory polypeptide (GIP) promoter into hamster insulinoma (HIT T15) cells indicated that the region between −180 and +14 is sufficient for basal promoter activity. Two CRE-BP1 binding sites were identified in this promoter region by DNase I footprinting with the bacterially expressed cAMP response element (CRE) binding protein, CRE-BP1. Mutation analyses showed that these two CREs are required for the basal promoter activity, and furthermore that one site, at nucleotide-158, contributed mainly to the cAMP inducibility of the GIP promoter in HIT T15 cells. Interestingly, the GIP promoter activity was repressed by the c-jun proto-oncogene product, possibly through the CREs.

【 授权许可】

Unknown