Naturally occurring deuterium is essential for the normal growth rate of cells

FEBS Letters

Lakics, Viktor¹  Jancsó, Gábor³  Vass, Kornélia²  Barna, Balázs⁴  Gaál, Tamás¹  Somlyai, Gábor²  Jákli, György³ 

[1] Chemical Works of Gedeon Richter Ltd., H-1475 Budapest, Hungary;HYD Ltd. for Research and Development, H-1215 Budapest, Hungary;Central Research Institute for Physics, H-1525 Budapest, Hungary;Plant Protection Institute, H-1525 Budapest, Hungary

关键词: Deuterium-depleted water;    Na+H+ antiport system;    Isotope effect;    Hydrogen isotope discrimination;    Cell growth;    DMEM;    Dulbeco's modified Eagle's media;    XTT;    2;    3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium-hydroxide;   

DOI  :  10.1016/0014-5793(93)81479-J

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The role of naturally occurring D in living organisms has been examined by using deuterium-depleted water (30–40 ppm D) instead of water containing the natural abundance of D (150 ppm). The deuterium-depleted water significantly decreased the growth rate of the L₉₂₉ fibroblast cell line, and also inhibited the tumor growth in xenotransplanted mice. Eighty days after transplantation in 10 (59%) out of 17 tumorous mice the tumor, after having grown, regressed and then disappeared. We suggest that the naturally occurring D has a central role in signal transduction involved in cell cycle regulation.

【 授权许可】

Unknown