In vitro complex formation between cholesterol and α1‐proteinase inhibitor

FEBS Letters

Eriksson, Sten¹  Janciauskiene, Sabina¹ 

[1] Gastroenterology and Hepatology Division, Department of Medicine. Malmö General Hospital, University of Lund, S-21401 Malmö, Sweden

关键词: α1-Proteinase inhibitor;    Cholesterol;    Complex formation;   

DOI  :  10.1016/0014-5793(93)81306-K

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The in vitro interaction between human (α₁-proteinase inhibitor (α₁-PI) and cholesterol was studied with electrophoretic and gel Chromatographic methods. The addition of cholesterol (from 1 to 20 mol/mol α₁-PI) at 37°C resulted in retarded electrophoretic mobility of α₁-PI towards the anode, diminished immunoreactivity and antiproteinase activity. At a molar ratio of 2:1 (cholesterol/α₁ -PI), antitryptic activity was reduced by 15% but antielastase activity by 50%. At this ratio the gel filtration α₁-PI peak appeared at 67 kDa, as compared to 52 kDa for native α₁-pi. No size difference was noted on SDS-PAGE. These results suggest the occurrence of noncovalent complex formation between cholesterol and α₁-PI in vitro.

【 授权许可】

Unknown