Mosquito DNA polymerase ε

FEBS Letters

Fuchs, Morton S.¹  Lee, Seung-Koo¹ 

[1] Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA

关键词: DNA polymerase ϵ;    3'-5' Exonuclease;    PCNA;    Mosquito;    Aedes aegypti;   

DOI  :  10.1016/0014-5793(93)81304-I

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The mosquito homolog of mammalian DNA polymerase ϵ, formerly known as a proliferating cell nuclear antigen (PCNA)-independent form of DNA polymerase δ, has been purified from mosquito larval extracts. The polymerase ϵ was separated from DNA polymerase α by chromatography on hydroxylapatite, and the enzyme was subsequently purified on single-stranded DNA agarose, followed by a 5' AMP-agarose chromatography step. The purified polymerase exhibits an intrinsic 3'-5' exonuclease activity and shows high activity using an oligo-primed DNA template. Neither human nor Drosophila PCNA stimulated this polymerase activity. Additional immunochemical and biochemical evidence indicates that this enzyme is distinct from DNA polymerase α.

【 授权许可】

Unknown