Conserved secondary structures in the ITS2 of trematode pre‐rRNA

FEBS Letters

Despres, Laurence¹  Bonhomme, François¹  Bachellerie, Jean-Pierre²  Michot, Bernard² 

[1] URA CNRS 1493, Laboratoire Gémme et Populations, Univ. Montpellier II, 34095 Montpellier Cédex, France;Laboratoire de Biologie Moléculaire Eucaryote, Institut de Biologie Cellulaire et de Génétique du CNRS, Université Paul Sabatier, 118 Route de Narbonne, 31062 Toulouse Cédex, France,

关键词: Pre-rRNA;    ITS2;    Secondary structure;    Trematode;    Evolution;    rRNA processing;    ITS2;    internal transcribed spacer 2;    The novel Fasciola hepatica sequence is available under the Genbank accession number L07844. The previously reported schistosome sequences are also available;    for S. mansoni (L03658) S. haematobium (L03656);    S. intercalatum;    a sequence identical to S. bovis (L03657);    and S. japonicum (L03660).;   

DOI  :  10.1016/0014-5793(93)81301-F

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Biological functions of transcribed spacer regions in eucaryotic pre-rRNAs remain elusive so far. Utilization of the comparative approach to analyse their secondary structure has been impeded by the extensive sequence divergence observed among most of the specimens available to date. However, we have taken advantage of the recent derivation of a set of largely similar sequences for different schistosome species to look for the presence of constrained secondary structures within the internally transcribed spacer 2 (ITS2). We show that several common features of secondary structure are shared by these species despite sequence variation, with some of them supported by compensatory changes, suggesting a significant role of ITS2 as an RNA domain during ribosome biogenesis.

【 授权许可】

Unknown