FEBS Letters | |
Identification and characterization of an anti‐tyrosine kinase factor in cystic gliomas | |
Benabid, A.L.1  Verna, J.M.1  Amalfitano, G.1  Brunet, J.F.1  Berger, F.1  Laine, M.1  Nissou, M.F.1  | |
[1] INSERM U. 318, CHU Grenoble, 38000 Grenoble BP 217X, France | |
关键词: EGF-R; Gliomas; Cystic fluid; Proto-oncogene; Tyrosine kinase; Inhibitory effect; | |
DOI : 10.1016/0014-5793(93)81204-D | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
In view of the frequent activation of the epidermal growth factor receptor (EGF-R) in gliomas and autocrine hypothesis, we searched for ‘EGF-like’ factor(s) in cystic fluids (CFs) associated with gliomas. Membranes of A431 cells, which overexpress EGF-R, were used to explore such activity in 20 CFs. In all cases CFs induced inhibition of EGF-R phosphorylation. Biochemical analysis revealed an anti-tyrosine kinase activity which was identified as a 18 kDa proteic factor. Effectiveness at high dilution and anti-proliferative effect on living cells in culture suggest that this factor may be involved in the negative regulation of glial oncogenesis.
【 授权许可】
Unknown
【 预 览 】
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RO201912020297393ZK.pdf | 497KB | download |