Identification and characterization of an anti‐tyrosine kinase factor in cystic gliomas

FEBS Letters

Benabid, A.L.¹  Verna, J.M.¹  Amalfitano, G.¹  Brunet, J.F.¹  Berger, F.¹  Laine, M.¹  Nissou, M.F.¹ 

[1] INSERM U. 318, CHU Grenoble, 38000 Grenoble BP 217X, France

关键词: EGF-R;    Gliomas;    Cystic fluid;    Proto-oncogene;    Tyrosine kinase;    Inhibitory effect;   

DOI  :  10.1016/0014-5793(93)81204-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In view of the frequent activation of the epidermal growth factor receptor (EGF-R) in gliomas and autocrine hypothesis, we searched for ‘EGF-like’ factor(s) in cystic fluids (CFs) associated with gliomas. Membranes of A431 cells, which overexpress EGF-R, were used to explore such activity in 20 CFs. In all cases CFs induced inhibition of EGF-R phosphorylation. Biochemical analysis revealed an anti-tyrosine kinase activity which was identified as a 18 kDa proteic factor. Effectiveness at high dilution and anti-proliferative effect on living cells in culture suggest that this factor may be involved in the negative regulation of glial oncogenesis.

【 授权许可】

Unknown