Regulation of Cu,Zn‐ and Mn‐superoxide dismutase transcription in Saccharomyces cerevisiae

FEBS Letters

Labbe-Bois, Rosine¹  Galiazzo, Francesca¹ 

[1]Institut Jacques Monod, Laboratoire de Biochimie des Porphyrines, Université Paris 7, 2 place Jussieu, 75251 Paris Cedex 05, France

关键词: Superoxide dismutase;    Paraquat;    Oxygen and heme;    Transcriptional regulation;    Saccharomyces cerevisiae;   

DOI  :  10.1016/0014-5793(93)81162-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The regulation of Cu,Zn- and Mn-superoxide dismutases (SOD) was investigated by Northern blotting and gene fusions of SOD1 and S0D2 promoters with the β-galactosidase reporter gene. Cu,ZnSOD expression was increased 3-fold under glucose derepressing conditions, and decreased 4- to 6-fold by oxygen or heme deficiency. MnSOD expression was increased 5-fold by glucose derepression, and decreased 8- to 10-fold by anaerobiosis and 4- to 5-fold by heme deficiency. Induction by paraquat was modest, about 50% for SOD1 and 100% for SOD2; it was apparently independent of the respiratory chain function.

【 授权许可】

Unknown