Defensin‐6 mRNA in human Paneth cells: implications for antimicrobia peptides in host defense of the human bowel

FEBS Letters

Bevins, Charles L.¹  Jones, Douglas E.² 

[1]The Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA

[2]The Division of Human Genetics and Molecular Biology, The Children's Hospital of Philadelphia, Philadelphia, PA, USA

关键词: cDNA;    Epithelium;    Crypt;    Intestine;    Mucosa;    cDNA;    complementary DNA;    HD-5;    human defensin-5;    HD-6;    human defensin-6;    HNP;    human neutrophil antimicrobial peptide;    mRNA;    messenger RNA;    PCR;    polymerase chain reaction;    SDS;    sodium dodecyl sulfate;    SSC;    standard sodium citrate (1 × concentration: 0.15 M NaCl;    0.15 M Na-citrate pH 7.0);   

DOI  :  10.1016/0014-5793(93)81160-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The epithelial surface of the human small intestine is a barrier between the host and the microbial environment of the lumen. A human small intestine cDNA clone was found to encode a new member of the defensin family of antimicrobial peptides, named human defensin-6. Tissue expression of this mRNA is specific for the small intestine as determined by Northern blot analysis and polymerase chain reaction analysis. In situ hybridization demonstrated that human defensin-6 mRNA localizes to Paneth cells in the crypts of Lieberkühn. The finding of an abundant defensin mRNA in human Paneth cells supports the notion that these epithelial cells may play a key role in host defense of the human bowel. The results also strengthen the hypothesis that peptide-based host defenses are prevalent at mucosal surfaces in mammals.

【 授权许可】

Unknown