Identification of toxigenic Vibrio cholerae from the Argentine outbreak by PCR for ctx A1 and ctx A2‐B

FEBS Letters

Herrmann, Patricio⁴  Varela, Pablo²  Frasch, Alberto C.C.¹  Binsztein, Norma³  Ugalde, Rodolfo A.¹  Cremona, Maria Laura²  Burrone, Oscar⁵  Rivas, Marta³ 

[1] Institute de Investigaciones Bioquimicas Fundacion Campomar, Antonio Machado 151, (1404) Buenos Aires, Argentina;Tecnologia Genetica, Buenos Aires, Argentina;Institute Nacional de Microbiologia ‘Carlos G. Malbran’, Avenida Velez Sarsfield 563, (1281) Buenos Aires, Argentina;Laboratorios BioCiencia, Buenos Aires, Argentina;International Centre for Genetic Engineering and Biotechnology, Padriciano 99, 34012 Trieste, Italy

关键词: Vibrio cholerae;    Toxin gene;    Polymerase chain reaction;   

DOI  :  10.1016/0014-5793(93)81136-N

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A polymerase chain reaction (PCR) to detect a region of the A1 cholera toxin gene was applied to the identification of 43 Vibrio cholerae strains isolated from the recent outbreak in Argentina. A good correlation was observed between the GM1-enzyme-linked immunosorbent assay (GM1-ELISA) to detect the B subunit of the enterotoxin and PCR. However, a V. cholerae non-01 strain that was negative by the ELISA test, was positive by the PCR assay for the Al region. A second PCR test to detect the A2-B coding region was developed to solve this case. We propose that routine detection of toxigenic V. cholerae by PCR should include analysis of A2-B coding region or the whole cholera toxin operon.

【 授权许可】

Unknown