Presence and coding properties of 2′‐O‐methyl‐5‐carbamoylmethyluridine (ncm5Um) in the wobble position of the anticodon of tRNALeu (U*AA) from brewer's yeast

FEBS Letters

Keith, Gérard¹  Desgrès, Jean³  Sochacka, Elzbieta³  El Adlouni, Chakib¹  Santos, Manuel²  Glasser, Anne-Lise³  Malkiewicz, Andrzej⁴  Tuite, Mick F.² 

[1] Institut de Biologie Moléculaire et Cellulaire du CNRS et Université Louis Pasteur, 67084 Strasbourg, France;Biological Laboratory, The University, Canterbury, Kent CT2 7NJ, UK;Laboratoire de Biochemie Médicale, Faculté de Médicine, Université de Bourgogne, 21034 Dijon, France;Institute of Organic Chemistry, Technical University (Politechnika), 90-924 Lodz, Zwirki 36, Poland

关键词: tRNALeu (U*AA);    Modified nucleoside;    Identification;    Mass spectrometry;    Anticodon;    Yeast;    ncm5Um;    2−O-methyl-5-carbamoylmethyluridine;    cm5Um;    2′-O-methyl-5-carboxymethyluridine;    ncm5U;    5-carbamoylmethyluridine;    BAP;    bacterial alkaline phosphatase;    SV-PDE;    snake venom phosphodiesterase;    (TMS) derivative;    trimethylsilyl derivative;    EI-MS;    electron impact mass spectrometry;    HPLC;    high performance liquid chromatography;    NMR;    nuclear magnetic resonance;   

DOI  :  10.1016/0014-5793(92)81510-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The unknown modified nucleoside U* has been isolated by enzymatic and HPLC protocols from tRNA^Leu(U*AA) recently discovered in brewer's yeast. The pure U* nucleoside has been characterized by electron impact mass spectroscopy, and comparison of its chromatographic and UV-absorption properties with those of appropriate synthetic compounds. The structure of U* was established as 2′-O-methyl-5-carbamoylmethyluridine (ncm⁵Um). The yeast tRNA^Leu (U*AA) is the only tRNA so far sequenced which has been shown to contain ncm⁵Um. The location of such a modified uridine at the first position of the anticodon restricts the decoding property to A of the leucine UUA codon.

【 授权许可】

Unknown