Pulsed magnetic field effects on calcium signaling in lymphocytes: Dependence on cell status and field intensity

FEBS Letters

Walleczek, Jan¹  Budinger, Thomas F.¹ 

[1] Center for Functional Imaging, Lawrence Berkeley Laboratory, University of California, Berkeley, CA 94720, USA

关键词: Calcium;    Immune system;    Signal Transduction;    ELF Electromagnetic Field;    Radical Pair Mechanism;    Rat thymic lymphocyte;    EMF;    electromagnetic field;    ELF;    extremely-low-frequency;    Con A;    concanavalin A;    B;    magnetic flux density;    E;    electric field intensity;    J;    current density;    [Ca2+]1;    cytoplasmic free calcium concentration;    AC;    alternating current;    DC;    direct current;   

DOI  :  10.1016/0014-5793(92)81504-F

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of 3-Hz, monopolar, quasi-rectangular magnetic field pulses on ⁴⁵Ca²⁺ uptake in resting and mitogen-treated rat thymic lymphocytes was evaluated. A 30-min, non-thermal exposure to the pulsed magnetic field (B _peak = 6.5 mT, E _max = 0.69 mV/cm, J _max = 2.6 μA/cm²) reduced Concanavalin A-induced ⁴⁵Ca₂₊ uptake by 45%. It was observed that (i) the induction of the 3-Hz field response dependend on Ca²⁺ signal transduction activation; (ii) the response direction (stimulation or inhibition) depended on the level of lymphocyte mitogen responsiveness, and (iii) the field response magnitude increased with increasing magnetic field flux densities (B _peak = 0, 1.6, 6.5 and 28 mT). Our results demonstrate field effects at B _max nearly 10⁴ greater than that of the average human environment for low-frequency magnetic fields and they are consistent with the independent results from other 3-Hz pulsed magnetic field studies with lymphocytes.

【 授权许可】

Unknown