DNA‐specific antiidiotypic antibodies in the sera of patients with autoimmune diseases

FEBS Letters

Gololobov, Gennady V.¹  Belostotskaya, Ksenia M.¹  Gromova, Irina I.¹  Alekberova, Zemfira S.²  Bronshtein, Igor B.¹  Kvashuk, Oksana A.¹  Gabibov, Alexander G.¹  Prokaeva, Tatiana B.²  Shuster, Alexander M.¹ 

[1] Engelhardt Institute of Molecular Biology, Academy of Sciences of Russia, Vavilov str. 32, 117984, Moscow B-334, Russia;Institute of Rheumatology, Academy of Medical Science of Russia, Moscow, Russia

关键词: Autoimmunity;    Topoisomerase I;    Antiidiotypic antibody;    Fusion protein;    HPLC of autoimmune sera;    Scl;    scleroderma;    SLE;    systemic lupus erythematosus;    RA;    rheumatoid arthritis;    MAB;    monoclonal antibodies against topoisomerase;    PAGE;    polyacrylamide gel electrophoresis;    HPLC;    high performance liquid chromatography;   

DOI  :  10.1016/0014-5793(92)81484-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Blood sera of patients with autoimmune diseases scleroderma (Scl), systemic lupus erythematosus (SLE), and rheumatoid arthritis (RA) have been shown to yield a specific immune response to topoisomerase I, the product of expression of a cDNA fragment cloned into λgt11 and monoclonal antibodies (MAB) to the enzyme. The ‘topoisomerase test’ is not absolutely specific for Scl. The stable positive response of autoimmune sera to anti-topoisomerase monoclonal antibodies has a specific character and is associated with the interaction of the Fab fragment of MAB with the IgG fraction of autoimmune serum. The response observed indicates the induction of anti-idiotypic antibodies against topoisomerase. The anti-idiotype, isolated by HPLC and affinity chromatography demonstrated the following functional activities: (i) the immunological reaction against DNA; (ii) high-affinity DNA-binding with topoisomerase-specific consensus; (iii) ability to compete with the native enzyme for binding with DNA and MAB to topoisomerase; (iv) immunological reaction against MAB to topoisomerase.

【 授权许可】

Unknown