Anticodon‐dependent aminoacylation of RNA minisubstrate by lysyl‐tRNA synthetase

FEBS Letters

Gladilin, K.L.¹  Wolfson, A.D.¹  Khvorova, A.M.¹  Motorin, Yu.A.¹ 

[1] A.N. Bakh Institute of Biochemistry, Russian Academy of Sciences, Leninsky pr. 33, 117071 Moscow, Russian Federation

关键词: Lysyl-tRNA synthetase;    RNA-minisubstrate;    tRNA recognition;   

DOI  :  10.1016/0014-5793(92)81483-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Specific inhibition of mammalian lysyl-tRNA synthetase by polyU is shown. Inhibition of the enzyme is dependent on the length of the oligonucleotide, since oligoU molecules with a length of less than 8 residues do not inhibit the aminoacylation, whilst the effect of oligoU molecules with a length of about 30 residues is the same as that of polyU. Inhibition is a result of recognition by the enzyme of the tRNA^Lys anticodon sequence (UUU) coded by polyU. Aminoacylation of the oligoU molecule with attached CCA sequence (G(U)₂₀-CCA) by yeast and mammalian lysyl-tRNA synthetases is demonstrated.

【 授权许可】

Unknown