FEBS Letters | |
Anticodon‐dependent aminoacylation of RNA minisubstrate by lysyl‐tRNA synthetase | |
Gladilin, K.L.1  Wolfson, A.D.1  Khvorova, A.M.1  Motorin, Yu.A.1  | |
[1] A.N. Bakh Institute of Biochemistry, Russian Academy of Sciences, Leninsky pr. 33, 117071 Moscow, Russian Federation | |
关键词: Lysyl-tRNA synthetase; RNA-minisubstrate; tRNA recognition; | |
DOI : 10.1016/0014-5793(92)81483-3 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Specific inhibition of mammalian lysyl-tRNA synthetase by polyU is shown. Inhibition of the enzyme is dependent on the length of the oligonucleotide, since oligoU molecules with a length of less than 8 residues do not inhibit the aminoacylation, whilst the effect of oligoU molecules with a length of about 30 residues is the same as that of polyU. Inhibition is a result of recognition by the enzyme of the tRNALys anticodon sequence (UUU) coded by polyU. Aminoacylation of the oligoU molecule with attached CCA sequence (G(U)20-CCA) by yeast and mammalian lysyl-tRNA synthetases is demonstrated.
【 授权许可】
Unknown
【 预 览 】
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